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. 2003 Sep 15;100(20):11789–11794. doi: 10.1073/pnas.1333899100

Fig. 2.

Fig. 2.

Ethylene and ROS involvement in Nod factor responses and nodulation. (A–C) Methylene blue-stained lateral root bases, observed 5 days postinoculation: control (A), treated with 10–9 M Nod factors (B), and with ethylene (C). (D) Transverse toluidine blue-stained section through a lateral root base inoculated with ORS571 after pretreatment with Ag+.(E) Root pretreated with 7 μM AVG, inoculated with ORS571(pRG960SD-32), 6 days after inoculation (arrow indicates bacteria viewed as blue GUS precipitate). (F) Toluidine blue-stained longitudinal section through E. (G) Influence of Ag+ on nodule initiation; roots were not treated (diamond) or were treated with Ag+ 2 days before (small square), 1 day before (triangle), simultaneously with (large square), 1 day after (asterisk), or 2 days after (circle) inoculation with ORS571. Note that the small square and triangle are masked by the large square. For abbreviations, see Fig. 1; vb, vascular bundle. [Bars = 100 μm (A–C and F) and 1 mm (D and E).]