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. 2003 Sep 19;100(20):11795–11800. doi: 10.1073/pnas.2032704100

Fig. 1.

Fig. 1.

Differential expression of ZMK1 and coleoptile bending angles during gravi- and phototropism of the maize coleoptile. (a) Relative transcript level (RU, relative units) of ZMK1 in light-exposed (open bars) and shaded (solid bars) coleoptile halves after 0, 30, 60, and 90 min of stimulation with unilateral blue light. The mRNA content (n = 3 ± SE) was quantified as described in Materials and Methods. The level of ZMK1 mRNA in the light-exposed half at t = 0 min was set to 1.0. (b) Curvature angle of coleoptiles after 4 hr of gravistimulation (g, black bar) and stimulation with unilateral blue light (bl, gray bar) and of photostimulated seedlings rotating on the BLC (bl-g, open bar). Bending angles (mean of n ≥ 30 ± SE) were measured as described in Materials and Methods. (c) Relative transcript level (RU) of ZMK1 in light-exposed (open bars) and shaded (closed bars) coleoptile halves of seedlings rotating on the BLC after 0, 30, 60, and 90 min of simultaneous stimulation with unilateral blue light. Blue light illumination started 30 min after the onset of clinostat rotation (–30). The mRNA content (n = 3 ± SE) was quantified as described in Materials and Methods. The level of ZMK1 mRNA in the light-exposed half at t = 0 min was set to 1.0.