Figure 2.

β-Neuregulin treatment has no effect on voltage-activated Ca²⁺ currents of CG neurons. Cells were isolated at E9 and cultured for 12 hr in the absence (A) or presence (B) of 1 nM β-neuregulin peptide. Recordings were made as described in Fig. 1 except that recording pipettes contained CsCl. Currents were evoked in the presence and absence of external Ca²⁺ (Left), and net voltage-activated Ca²⁺ currents were then obtained by digital subtraction (Right). (C) Mean Ca²⁺ current density is not affected by β-neuregulin treatment.