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. 1997 May 27;94(11):5943–5948. doi: 10.1073/pnas.94.11.5943

Figure 1.

Figure 1

PCR products with isoform-specific primers and reticulocyte single-stranded cDNA as template. Photograph of PCR products that were separated in a 2% agarose gel. The following conditions were used: 40 cycles; denaturation for 20 s at 98°C; annealing and extension for 90 s at 65°C or annealing at 60°C for 1 min and extension at 72°C for 1 min; ≈30 ng of reticulocyte single-stranded cDNA as template. The specific primer pairs used below are defined in Table 1. Lanes L show a 1-kb mass ladder (Life Technologies). (Left) Lanes: 1, α1-specific primer pair A1-1 and A1-2, expected product of 530 bp; 2, α2-specific primer pair A2-1 and A2-2, expected product of 612 bp; 3, α3-specific primer pair A3-1 and A3-2, expected product of 540 bp; 4: α4-specific primer pair A4-1 and A4-2, expected product of 494 bp; 5, β1-specific primer pair B1-1 and B1-2, expected product of 441 bp; 6, β2-specific primer pair B2-1 and B2-2, expected product of 455 bp; 7, β3-specific primer pair B3-1 and B3-2, expected product of 414 bp. (Right) Lanes L and 8 are from a 3% gel. Lanes: L, 1-kb mass ladder; 8, γ-specific primer pair γ1 and γ2, expected size 186 bp. See text for discussion.