Figure 4.

Amiloride-sensitive current in isoforms of XENaC. Oocytes injected with the indicated combination of cRNA α, β, and γ subunits were studied under voltage-clamp conditions at −100 mV in a 100 mM sodium amphibian Ringer. (a) The current inhibited by a high concentration of amiloride (20 or 50 μM). The number of observations were 41, 25, 23, and 18 for the αβγ, αβ2γ, αβγ2, and αβ2γ2 groups, respectively. (b) The inhibitory constant of amiloride in each group. The number of measurements were 10, 4, 7, and 8 in αβγ, αβ2γ, αβγ2, and αβ2γ2, respectively.