Figure 7.

Amiloride affinity in γ/γ2 chimeric constructs. The inhibition constant (K_I) for amiloride was measured in oocytes injected with α and β subunit cRNA and either γ, γ2, or one of the four chimeric constructs cRNA. The scheme shows the structure of the chimera with the γ part hatched and the γ2 part shaded. The position of the two hydrophobic segments is indicated by solid boxes. The statistical significance of the differences is indicated as follows: ∗ and ∗∗ indicate P < 0.01 and P < 0.001, respectively, when compared with the γ group. • and •• indicate P < 0.01 and P < 0.001, respectively, when compared  ith the γ2 group.