Figure 4.

Total RNA gel blot analysis of LeExp1 expression in three expanding stages of fruit (I–III), and fruit ripened on the vine (Top) or first harvested at the MG stage and allowed to ripen in the presence of air (Middle) or ethylene at 10 μl/liter (Bottom). Ripening stages included immature green (IG), MG, breaker (BR), turning (TU), pink (PI), light red (LR), and red ripe (RR). Each blot was hybridized with probe 2 and exposed to film at −80°C for approximately 9 h.