Figure 5.

Effect of NBD on LeExp1 expression. MG fruit were harvested and held in air, NBD at 2 ml/liter, or the same NBD concentration plus ethylene at 10 μl/liter until air-treated control fruit reached the breaker plus 4 day or red ripe plus 4 day (over ripe) stages. Fruit from all three treatments were used to isolate total RNA (15 μg per lane) for RNA gel blot analysis using probe 2.