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RNA gel blot analysis of LeExp1 expression in fruit ripening series of wild-type fruit (Ailsa Craig) and equivalent-age rin, nor, and Nr mutant fruit. Total RNA was isolated from MG, breaker (BR), red ripe (RR), or MG fruit treated with ethylene at 20 μl/liter for 8 h (MG/E), separated by gel electrophoresis (15 μg per lane), and hybridized with probe 2. This blot was generously provided by J. Giovannoni and P. Kannan (Texas A&M University).
