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. 1997 May 27;94(11):5961–5966. doi: 10.1073/pnas.94.11.5961

Figure 6.

Figure 6

Analysis of the DNA-binding properties of AtEBP. (A) Analysis of AtEBP binding to the GCC wild-type (wt) sequence from the tobacco PRB-1b promoter (−201 to −191) and a GCC mutant (m) sequence. Lanes 1 and 5 contained only the free probes. Lanes 2 and 6 contained 5 ng, lanes 3 and 7 contained 25 ng, and lanes 4 and 8 contained 50 ng of AtEBP. Lanes 9 and 10 contained 100 ng of GST–AtEBP. (B) The oligonucleotide sequences for the GCC wild-type and mutant probes. The mutated residues in the GCC mutant probe are outlined. The 5′ end of each probe contained a BamHI site.