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. 1997 May 27;94(11):5961–5966. doi: 10.1073/pnas.94.11.5961

Figure 7.

Figure 7

DNase I footprint analysis of AtEBP binding to the GCC box in the PRB-1b promoter. A PRB-1b promoter fragment corresponding to nucleotides −205 to −155 was labeled on both strands (bottom, top) and incubated with no protein (lanes 2, 5, 7, and 10), 50 ng AtEBP (1x; lanes 3 and 8), or 100 ng AtEBP (2x; lanes 4 and 9). The reactions were analyzed on a 8% sequencing gel together with G+A sequencing ladders generated from the same PRB-1b promoter fragments. The GCC box on the PRB-1b promoter is indicated by arrows.