Table 3.
Nucleotide sequences of the primers sets used for RT-PCR assays. The rest of the primer sets has been already reported [18].
| Gene | Primer | Primer sequence |
| Aromatic amino acid pathways | ||
| aroF | aroFa | 5' CAGAATCCGTGGTTGCTCAA 3' |
| aroFb | 5' CGGTTGCTCGGAAGACTGAT 3' | |
| aroG | aroGa | 5' GTCGGCTTCAAAAATGGCAC 3' |
| aroGb | 5' CCCCATTTCGTTACGGACAG 3' | |
| aroH | aroHa | 5' CTGCTCGATCCACGATCTCA 3' |
| aroHb | 5' AGGTGCGCATTACGATTTCC 3' | |
| aroB | aroBa | 5' GTTATCCTCCCTGACGGCG 3' |
| aroBb | 5' ACCAGCGTAGTATCGCGACC 3' | |
| aroD | aroDa | 5' AGCCGAAGAAATCATTGCCC 3' |
| aroDb | 5' ACGTCAGCACATCGCTGGTA 3' | |
| aroE | aroEa | 5' AATCCGATAGCCCACAGCAA 3' |
| aroEb | 5' TCATTGATGGGTGCCAACAC 3' | |
| ydiB | ydiBa | 5' AAAAGCGGGATTGCCATTTA 3' |
| ydiBb | 5' CAGTTCCGCGCATTTTGAG 3' | |
| aroL | aroLa | 5' CCGGAAGAAGATTTACGGCC 3' |
| aroLb | 5' TGCGCAACTTCGCGATATAG 3' | |
| aroK | aroKa | 5' GCACTATTGGGCGCCAGTTA 3' |
| aroKb | 5' GAAAACCCAGCCCACATCAG 3' | |
| aroM | aroMa | 5' CCAGAAGCAGGCGAAGACAC 3' |
| aroMb | 5' TCAACCACACCTTGCAGGTC 3' | |
| aroA | aroAa | 5' CATATTCCTGATGCGGCGAT 3' |
| aroAb | 5' TCGGTCTCTTTAACACGCCAG 3' | |
| aroC | aroCa | 5' GGGACATCGCGCTATACCAC 3' |
| aroCb | 5' AACAAGCCAATGCTGGTGC 3' | |
| pheA | pheARa | 5' ATGACCCGTCTGGAATCACG 3' |
| pheARb | 5' GCTTTTTGCATTTCCGCTG 3' | |
| pheA-ev2 | pheAa | 5' ACTCAGCAGGCTTTGCTCCA 3' |
| pheAb | 5' GCCGCAAGATGGGAATAAGAA 3' | |
| tyrA | tyrAa | 5' GTCAGATGGGACGCCTGTTC 3' |
| tyrAb | 5' GGCAACAATATCAGCCGCTC 3' | |
| aspC | aspCa | 5' GGCGATTCGCGCTAACTACT 3' |
| aspCb | 5' GCTCTTGTTCCCAAATCGCA 3' | |
| trpE | trpEa | 5' CGCAACTGTCAGCGGAAAAT 3' |
| trpEb | 5' GGCCTGAATACGGGTGCTTT 3' | |
| trpA | trpAa | 5' CGTTCCTTTCGTCACGCTC 3' |
| trpAb | 5' CGTTCCTTTCGTCACGCTC 3' | |
| Aromatic amino acid transport | ||
| pheP | phePa | 5' CGGCTGTATTGCTGGGCTAC 3' |
| phePb | 5' AAATGAACCGGATACCGGCT 3' | |
| aroP | aroPa | 5' TGGTAATCAACTGGGCGATG 3' |
| aroPb | 5' CGGATAAAGCAGAGCAGGGA 3' | |
| tyrP | tyrPa | 5' CCGGTGTTGGTTTTAGCGTT 3' |
| tyrPb | 5' CGGAACATGCTGGTACACCTC 3' | |
| mtr | mtra | 5' TTGATGTGCTGGTACAGGCG 3' |
| mtrb | 5' GAGGAACGAACTCGCTACCG 3' | |
| shiA | shiAb | 5' ATTATCAAGCTGCCGCGAAA 3' |
| shiAb | 5' CAATTCGCACAGTCGTAGCG 3' | |
| ilvE | ilvEa | 5' AAGGCATCCGTTGCTACGAC 3' |
| ilvEb | 5' CGGGAAGCGATAGATTTTGG 3' | |
| aroP | aroPa | 5' TGGTAATCAACTGGGCGATG 3' |
| aroPb | 5' CGGATAAAGCAGAGCAGGGA 3' | |
| Regulators | ||
| trpR | trpRa | 5' CAGAACAGCGTCACCAGGAG 3' |
| trpRb | 5' TGGCGTCAGCATCAGGTTTA 3' | |
| tyrR | tyrRa | 5' GGCGAGCTGTCAGCTTTTTG 3' |
| tyrRb | 5' CGCTTTCCAGCCAACGTAAA 3' | |
| csrA | csrAa | 5' TCTGACTCGTCGAGTTGGTGA 3' |
| csrAb | 5' GGGCATTTACGCCAATACGT 3' | |
| rpoD | rpoDa | 5' GATTCTGCGACCACCGAAAG 3' |
| rpoDb | 5' TCGATACCGAAACGCATACG 3' | |
| rpoE | rpoEa | 5' GAACTATTGAGTCCCTCCCGG 3' |
| rpoEb | 5' CGGACAATCCATGATAGCGG 3' | |
| fnr | fnra | 5' CGGAAAAGCGAATTATACGGC 3' |
| fnrb | 5' TTCGTTGAGTGTGAACGGGA 3' | |
| Carbohydrate transport | ||
| malE | malEa | 5' AGAAATTCCCACAGGTTGCG 3' |
| malEb | 5' TTCAGCCAACAGGCCAGATT 3' | |