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Caspase-3 assay in RGC-5 cells. Colorimetric analysis of the effects of agmatine on the caspase-3 activity induced by hypoxic injury in RGC-5 cells. Cells were exposed to hypoxia for 24 hours with or without 100 μM agmatine or caspase-3 inhibitor Z-VAD-FMK (50 μM). Specific activity of caspase-3 was measured by cleavage of the caspase-3 substrate Ac-DEVD-pNA.
