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. 1998 Jul 7;95(14):7860–7865. doi: 10.1073/pnas.95.14.7860

Figure 1.

Figure 1

Cassettes containing ThEn42 (genomic or cDNA) used for plant transformation. (A) CaMV35S-Pro = promoter; nopaline synthase (NOS) = transcription terminator; coding sequence for the fungal signal peptide (FSP), plant secretion peptide (PSP), fungal putative activation peptide (AP). (B) The 5′ noncoding region of p35S-psCHIT42 with PSP (pTE 28.1) and p35S-CHIT42 with FSP. Box indicates mRNA stabilization consensus sequence, and topline indicates A+T rich regions (20). Bold letters indicate consensus sequences for transcription in the ATG region compared with TAA ACA ATG GCT (33). (C) Elements reducing mRNA stability at the 3′ noncoding region of the fungal gene (33) are boxed or underlined. In p35S-psCHIT42, the region downstream from restriction site SspI (arrowhead) was removed.