Figure 3.

SMC attachment to collagen was reduced in the absence of DDR1. SMCs from DDR1+/+ mice (filled bars) or DDR1–/– mice (open bars) were allowed to attach for 4 hours to plates coated with 10 nM fibronectin or vitronectin or 100 nM type I collagen or type VIII collagen. Attachment was assessed by staining the cells with toluidine blue and measuring optical density at 595 nm. Experiments were done in triplicate and repeated three times. ^AValue from DDR1–/– SMCs was significantly less than the value from wild-type SMCs.