Figure 4.

Cell proliferation on collagen was less in DDR1–/– SMCs compared with DDR1+/+ SMCs. (a) SMCs from DDR1+/+ mice (filled bars) or DDR1–/– mice (open bars) were stimulated to grow with 10% FCS and incubated with [³H]thymidine on wells coated with 100 nM type I collagen (left) or type VIII collagen (right). ^AValue from DDR1–/– SMCs was significantly less than value from wild-type SMCs. (b) Number of SMCs per well after plating on 100 nM type I collagen and growing in DMEM containing 10% FCS for 1–4 days. Squares, DDR1+/+ SMCs; circles, DDR1–/– SMCs. Experiments were done in triplicate and repeated three times. ^AValue from DDR1–/– SMCs was significantly less than value from wild-type SMCs.