Figure 5.

SMC migration was reduced in the absence of DDR1. SMCs from DDR1+/+ mice (filled bars) or DDR1–/– mice (open bars) were stimulated to migrate for 4 hours in chemotaxis chambers with 200 nM type I collagen (left) or type VIII collagen (right) added to DMEM in the bottom of the chamber. Migration was quantified by staining and counting the number of cells that migrated to the bottom of the filter. Experiments were done in triplicate and repeated three times. ^AValues from DDR1–/– SMCs were significantly less than values from wild-type SMCs.