Figure 2.

The cDNA in p37 restores wild-type GSII phenotype of the UDP-GlcNAc Golgi transporter mutant. The cDNA in the selected p37 clone was extracted and religated, in either orientation, in the expression vector pE4 under the control of the PGK constitutive promoter. Upon transformation into the Golgi UDP-GlcNAc transporter-deficient mutant, the plasmid containing the cDNA in the plus orientation with respect to the promoter (PGN4+) restored the fluorescence profile resembling that of wild-type cells. Cells transformed with the plasmid in the minus orientation (PGN4-) showed no phenotypic correction.