Table 1.
Fraction | N-terminal sequence* | Molecular mass, Da
|
Peptide | |
---|---|---|---|---|
Observed | Calculated† (Hyp/Pro) | |||
H1 | Blocked | 5,434.0 | 5,434.7 (16/0) | ‡Gln1 –Ala57 |
H2 | TOAFAOSGGVAL | 2,716.2 | 2,711.9 (3/4) | Thr59 –Ala88 |
2,732.2 | 2,727.9 (4/3) | Thr59 –Ala88 | ||
H3 | Blocked | 8,261.2 | 8,259.7 (20/3) | ‡Gln1 –Ala88 |
Determined by N-terminal protein sequencing. O, Hyp.
Calculated with number of Hyp/Pro residues chosen to give a molecular mass most similar to the experimentally determined value.
Gln, pyroglutamate.