Figure 3.

Ligand-dependent interaction between TRα and TRAP220. (A) Far Western analysis. Five hundred nanograms of purified TR–TRAP complex was separated by SDS/PAGE (8% gel), blotted, and probed with ³⁵S-labeled full-length (TR) and C-terminal (TRα-C) hTRα in the presence and absence of 10⁻⁷ M 3,3′,5-triiodothyroacetic acid (T₃). Arrow indicates the position of the TRAP220 band. (B) Coimmunoprecipitation of TR-TRAP220 from in vitro translated products. The indicated combinations of ³⁵S-labeled TRAP220, TRAP100, and FLAG-TRα were incubated in the presence and absence of 3,3′,5-triiodothyroacetic acid (T₃), and subjected to immunoprecipitation with anti-FLAG antibodies (M2 agarose) that recognize only FLAG-tagged TRα.