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. 1998 Jul 7;95(14):7939–7944. doi: 10.1073/pnas.95.14.7939

Figure 4.

Figure 4

Nuclear receptor interactions with TRAP220 and TRAP100. (A) Ligand-dependent interactions of GST-TRAP220/1 and -2 with 35S-labeled TRα. GST or GST-TRAP220/1 and -2 fusion proteins (1.5 μg) were incubated with 35S-labeled TRα, TRα-C, or TRα-N in the presence and absence of 10−7 M 3,3′,5-triiodothyroacetic acid. (B) Interactions between GST receptors and 35S-labeled TRAP220 and TRAP100. GST and GST nuclear receptor fusion proteins (1.5 μg) were immobilized and incubated with [35S]TRAP220 or [35S]TRAP100 at either 300 mM KCl (TRα, VDR, RARα, and ERα) or at 100 mM KCl (RXR, PPARα, and mouse PPARγ). The cognate ligands were 10−7 M 3,3′,5-triiodothyroacetic acid (TRα), 10−6 M all-trans retinoic acid (RARα), 10−6 M 9-cis retinoic acid (RXRα), 10−7 M 1α,25(OH)2D3 (VDR), 10−4 M Wy14643 (PPARα), and 10−5 M 15-deoxy-D12,14-prostaglandin J2 (mouse PPARγ).