Figure 2.

A) Effect of NS-398 on PGE₂ production in P388D₁ cells. The cells were incubated with the indicated concentrations of NS-398 for 30 min before and during the LPS/PAF treatment. PGE₂ in the supernatants was quantitated by using a specific radioimmunoassy. Basal PGE₂ production—i.e., that observed in the absence of LPS/PAF at the indicated NS-398 concentrations—is indicated by the open circles. (B) Effect of COX inhibitors on PGE₂ production from exogenous AA. The cells were treated with 5 μM NS-398, 25 μM indomethacin (Indo), 25 μM aspirin (Aspi), or neither (Ctrl) for 30 min before and during the 1-h treatment with LPS. Afterward, 1 μM AA was added and the amount of PGE₂ produced after 10 min was quantitated by using a specific radioimmunoassay.