Figure 5.

AA restores the enhancing effect of exogenous sPLA₂ on [³H]AA mobilization in activated P388D₁ cells. [³H]AA-labeled LPS-treated cells were preincubated with MAFP (25 μM) for 15 min where indicated. Subsequently, 1 μM exogenous unlabeled AA was added 1 min before treatment with PAF (100 nM), as indicated. Where indicated, exogenous sPLA₂ (500 ng/ml) was added together with PAF. The data are expressed as a percentage of the response observed in activated cells in the absence of any addition, which was taken as 100%.