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Absence of dTTP hydrolysis by the primase fragment. Nucleotide hydrolysis assays were performed by using 5 mM [α-32P]dTTP as the substrate. Rates of nucleotide hydrolysis were determined for reactions containing various amounts of the 63-kDa gene 4 protein (•) or the primase fragment (○). Protein concentrations are reported in terms of protein monomers.
