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Effect template concentration on oligoribonucleotide synthesis. The products of oligonucleotide synthesis assays containing ATP and [α-32P]CTP were as described in Fig. 4. The rates of oligonucleotide synthesis were determined for reactions containing the indicated concentrations of 20-base oligonucleotide (3′-GGGTCX15) and either the primase fragment (○), the 63-kDa gene 4 protein (•), or the 63-kDa gene 4 protein in the presence of 1 mM dTTP (▴).
