Figure 7.

Interaction between the primase fragment and T7 DNA polymerase. The reaction mixtures contained 12 μg/ml M13, 100 μM CTP, 100 μM ATP, and 300 μM each of [α-³²P]dGTP, dATP, dCTP, and dTTP. After a preincubation at 30°C for 10 min, reactions were initiated by the addition of T7 DNA polymerase premixed with either the primase fragment or the 63-kDa gene 4 protein. Rates of incorporation of nucleotides into DNA are reported for reactions containing various amounts of the primase fragment (○) or the 63-kDa gene 4 protein (•).