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. 1998 Jul 7;95(14):7963–7968. doi: 10.1073/pnas.95.14.7963

Figure 1.

Figure 1

Map of pTSA-3′proc vector designed for gene expression in procyclic forms of T. brucei (kindly provided by D. Salmon and E. Pays). The coding sequences (PPDK: pyruvate, phosphate dikinase; HYG: hygromycine resistance; α-TUB and β-TUB: α- and β-tubulin) are indicated by hatched boxes. The black boxes flanking the PPDK gene contain the procyclin promoter indicated by an arrow (5′ proc) or the polyadenylation signal present in the 3′ noncoding region of the procyclin (3′ proc), and the splice leaders (SL) are shown. The white boxes represent the tubulin intergenic region used to target the insertion of the recombinant pTSA-3′proc (B) into the tubulin gene cluster (A), and the pUC18 vector is shown as a hatched thin box. Abbreviations: B, BssHII; Xb, XbaI and Xh, XhoI.