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Immunofluorescence analysis of T. brucei procyclic forms (EATRO 1125). Untransfected cells were stained with anti-aldolase (A) or anti-PPDK H121 (B) mAbs; anti-poly(His) was used to stain cells expressing histidine-tagged PPDK, which contains the AKL glycosomal targeting motif (PPDK34) (C) or histidine-tagged PPDK depleted of AKL glycosomal targeting motif (PPDK35) (D).
