Figure 5.
Western blot analysis of trypanosomatids. In A, lysates of 107 cells from T. brucei 427 procyclic forms (lane 1) and bloodstream forms (lane 2), T. congolense procyclic forms (lane 3), epimastigote forms (lane 4), and bloodstream forms (lane 5), T. vivax bloodstream forms (lane 6), T. cruzi epimastigote forms (lane 7), Phytomonas (lane 8), and C. fasciculata choanomastigote forms (lane 9) were probed with mouse polyclonal anti-PPDK. In B, lysates of 3 × 107 cells from T. brucei 427 procyclic forms (lane 1), T. brucei GuTat short–stumpy form (lane 2) and long–slender form (lane 3), L. amazonensis promastigote forms (lane 4), and amastigote forms (lane 5) were analyzed as in A.