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Nuclear microinjection of fluorescent SRP RNA mutant corresponding to the SRP9/14 binding portion of the Alu domain (ΔL21 in Fig. 4). (A) Phase-contrast image taken 1 min after microinjection. Fluorescence at 1 min (B), 3 min (C), and 16 min (D) after microinjection. (E and F) Phase-contrast and fluorescence of a noninjected cell. Note the same early, transient nucleolar localization as observed with full-length SRP RNA in Fig. 2.
