Skip to main content
. 1998 Jul 7;95(14):7981–7986. doi: 10.1073/pnas.95.14.7981

Figure 6.

Figure 6

SRP RNA mutant corresponding to the S domain (Δ35 in Fig. 4). Two representative experiments are shown, illustrating the extremely short-lived nucleolar localization with this S domain mutant. (A) 1 min after microinjection. (B) 3 min. (C) 24 min. (D) Phase-contrast at 24 min. Note that in contrast to wild-type SRP RNA (Fig. 2) or the Alu domain mutant (Fig. 4), which show nucleolar localization for at least 1–3 min after nuclear microinjection, the S domain mutant displays no nucleolar localization at 1 min (A). The pattern is a punctate nucleoplasmic distribution. (E–H) In this second experiment, nucleolar localization was observed at 30 sec after nuclear microinjection (E) but did not persist. (F) 3 min. (G) 10 min. (H) 19 min. (I) Phase-contrast at 19 min.