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. 2007 Dec 5;2(12):e1271. doi: 10.1371/journal.pone.0001271

Table 2. Insertion sequence (IS) elements identified within intergenic and flanking regions of serotype A and B strains.

Subtype Location1 Intergenic Space2 IS Family3 IS Group3 IS Homolog3 Present/Full-length4 %Present5
A1 upstream 963 IS3 IS150 IS 1233 82/177 46%
downstream 2185 IS3 IS150 ISSau2 210/521 40%
downstream 2185 IS3 IS150 IS 1069 216/272 79%
A2 orfX1/p21 1226 IS6 ISS1Z 234/340 68%
A3 upstream 1329 IS605 IS200 IS200S 56/151 37%
downstream 679 IS3 IS150 ISSau2 148/521 28%
orfX1/p21 1230 IS6 ISS1Z 254/340 75%
A4 orfX1/p21 1313 IS6 ISOur1 282/340 83%
bvB downstream 2791 IS256 ISCp1 94/383 25%
B1 downstream 2780 IS256 ISCp1 77/329 23%
1

IS element location with respect to the toxin complex.

2

Intergenic space (in nucleotides)

3

IS family, group and homolog identified using the IS Finder database.

4

The IS element showing greatest homology at the amino acid level using BLAST

5

Amino acids present/amino acids of the full-length element

IS elements present within the intergenic or flanking regions of the toxin complex from each subtype were identified and characterized by IS family and group. Only partial IS elements ranging from 23–83% homology to the full-length elements, were identified. The IS elements and their location are the same within the BoNT/A1 strains of ATCC 3502, ATCC 19397 and Hall but not shown.