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. Author manuscript; available in PMC: 2008 Mar 9.
Published in final edited form as: Cell. 2007 Mar 9;128(5):947–959. doi: 10.1016/j.cell.2007.01.042

Figure 2.

Figure 2

Microarray analysis. A. Fluorescence channels 1 (Fl1, 530 nM) and 2 (Fl2, 585 nM) FACS plots of wildtype (+/+), Nkx2-5gfp/+ heterozygous and Nkx2-5gfp/gfp null embryos (both axes log10 scale). Gates define propidium iodide-permeable dead cells (dashed line) and live Nkx2-5-GFP+ cells (solid line). Nkx2-5-GFP+ cells (green) were used for microarray analysis. B. Microarray heat map showing fold-change in mRNA expression between Nkx2-5 null and heterozygous GFP+ cells (see scale). Genes modified at least 1.7-fold at 3 embryonic stages were clustered by standard correlation analysis (see tree diagrams for genes, left, and for embryonic stages, above). Genes known to be down-regulated in Nkx2-5 mutants are arrowed. The prominent cluster of genes up-regulated from 6–21 ps is indicated (*).