Skip to main content
. Author manuscript; available in PMC: 2008 Mar 9.
Published in final edited form as: Cell. 2007 Mar 9;128(5):947–959. doi: 10.1016/j.cell.2007.01.042

Figure 5.

Figure 5

Modified expression of cardiac signaling components in Nkx2-5 null embryos. AD. Bmp2 expression in wildtype (+/+) and Nkx2-5gfp/gfp null embryos at E8.0 (A–C) and E8.5 (D). Elevated expression in mutants arrowed. E–I. Phospho-Smad1/5/8 expression at E7.75 (E,F) and E8.0 (G–I). Elevated expression in mutants indicated in cardiac mesoderm (single arrows), endoderm (arrowheads) and ventral neural tube (double arrows). J–L. Fgf10 expression at E7.5 (J), E8.5 (K) and E9.5 (L), arrows showing ectopic expression. M–P.β-galactosidase activity from the Mlc1v-nlacZ-24 transgene at E8.0 (M,N) and E9.5 (O,P), with arrows showing ectopic expression. Q,R. Co-immuno-detection of β-galactosidase protein from the Mlc1v-nlacZ-24 transgene (green nuclei) and sarcomeric myosin (red) at E7.5 in the cardiac crescent in Nkx2-5gfp/+ control and Nkx2-5gfp/gfp embryos. Arrows indicate expanded expression of β-galactosidase in myosin+ cardiomyocytes in mutants. S–V. Hhex expression at E7.75 showing elevated expression in cardiac endoderm (arrows) and endothelial cells within cranial mesenchyme (arrowheads) of Nkx2-5gfp/gfp embryos. W,X. Wnt11 expression (arrows) is severely reduced in Nkx-2-5 null embryos at E7.75 (Y) and E8.5 (Z).