Figure 5.

Immunolocalization of transgene-derived hIL-1α. Immunostaining of hIL-1α in knee joint sections showed positive staining in proliferating synovial lining cells (a, ×200) and large mononuclear cells in synovial fluid (b, ×320). Freshly isolated synoviocytes from 8-week-old Tg mice were analyzed for F4/80 and hIL-1α expression by flow cytometry (c). The levels of intracellular hIL-1α after gating on F4/80⁺ cells or F4/80^– cells are shown in the histograms. The percentage of cells producing hIL-1α is indicated. The data are representative of four separate experiments.