Figure 1.

Effects of chronic ET-1 treatment on insulin-stimulated 2-DOG uptake in 3T3-L1 adipocytes. (a) After treatment with or without ET-1 (10 nM) for 24 hours, serum-starved 3T3-L1 adipocytes were treated with ET-1 (10 nM) or insulin (3 or 100 ng/ml), followed by measurement of 2-DOG uptake. (b) After treatment with BQ 610 (1 μM) for 30 minutes or PTX (100 ng/ml) for 16 hours, 3T3-L1 adipocytes were treated with ET-1 (10 nM) for 24 hours, followed by measurement of insulin-stimulated (100 ng/ml) 2-DOG uptake as described in Methods. The data are mean ± SE from three independent experiments.