Figure 2.

Effects of chronic ET-1 treatment on insulin-stimulated GLUT4 translocation in 3T3-L1 adipocytes. (a) After treatment with or without ET-1 (10 nM) for 24 hours, serum-starved 3T3-L1 adipocytes on coverslips were treated with insulin (10 ng/ml), followed by measurement of GLUT4 translocation. (b) After treatment with BQ 610 (1 μM) for 30 minutes, PD98059 (50 μM) for 30 minutes, PTX (100 ng/ml) for 4 hours, or microinjection with GST-BARK mixed with sheep IgG, 3T3-L1 adipocytes on coverslips were incubated with ET-1 (10 nM) for 24 hours, followed by measurement of insulin (10 ng/ml) stimulated GLUT4 translocation as described in Methods. The data are mean ± SE from three independent experiments.