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. 2001 May 1;107(9):1153–1161. doi: 10.1172/JCI11846

Figure 5.

Figure 5

Reduced Twist dosage increases the formation of nodular structures by mutant calvaria osteoblasts. Control (Nl) and mutant (M-Tw) calvaria cells were plated at the same density in the presence of ascorbic acid to induce the formation of nodules. (a) Nodule formation (arrows in upper panel; ×125) and matrix formation (M in lower panel; ×250) is shown. The number of ALP+ nodules was determined at 8 days of culture (b). Data are the mean ± SEM of three to four cultures. AP < 0.01 vs. Nl cells.