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. 1998 Jul 7;95(14):8064–8069. doi: 10.1073/pnas.95.14.8064

Figure 1.

Figure 1

(a) Analysis of transfected L929 cell clones by immunoblot. (Top) Cell lines L929 (lane 1) and 11D6 (lane 2) probed with a pan-Dsc-specific antibody 52–3D and cell line AB5 probed with Dsc1b-specific antibody RO851 (lane 3), Dsc1a-specific antibody RO852 (lane 4), and 52–3D (lane 5). (Middle) Cell lines L929 (lane 1), 11D6 (lane 2), and AB5 (lane 3) probed with rabbit anti-PG. (Bottom) Cell lines L929 (lane 1), 11D6 (lane 2) and AB5 (lane 3) probed with a Dsg antibody 3a. Cell line 11D6 expresses low levels of Dsc1a and Dsc1b and high levels of Dsg1 and PG, whereas cell line AB5 expresses high levels of Dsc1a and Dsc1b and lower levels of Dsg1 and PG. (b) Analysis of transfected L929 cell clones by flow cytometry. AB5 and untransfected L929 cells were subjected to FACS analysis by using antibody HM6 against Dsca/Dscb (Upper) and antibody 3a against Dsg1 (Lower).