Figure 7.
Effect of IL-4–transduced DCs on T-cell cytokine pattern in spleen. Mice were immunized with CII on day 0. On day 15, mice received intraperitoneal injections of 3 × 105 mock-transduced (gray bars) or IL-4–transduced (filled bars) DCs. Control animals were given HBSS (open bars). On day 21, spleens were harvested. Cells were cultured for 72 hours with anti-CD3 (5 μg/ml) or for 96 hours in the presence of CII (100 μg/ml). Culture supernatants were analyzed for IFN-γ and IL-4 by ELISA. Data are the mean ± SEM of triplicate cultures. The data shown are from a single experiment representative of two identical experiments.