Figure 4.

Homocysteine induces the steady-state mRNA levels of genes involved in cholesterol biosynthesis in HepG2 cells. Northern blot analysis of the steady-state mRNA levels of IPP isomerase, HMG-CoA reductase, and FPP synthase in HepG2 cells cultured in the absence or presence of 5 mM homocysteine for the indicated time periods. Total RNA (10 μg/lane) was size fractionated by agarose-gel electrophoresis, transferred to nylon membranes, and subjected to blot hybridization using the appropriate radiolabeled cDNA probe. Control for equivalent RNA loading was assessed using a radiolabeled GAPDH cDNA probe.