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Effect of homocysteine on LDL uptake in HUVECs, HASMCs, and HepG2. Cells treated in the absence or presence of 5 mM homocysteine for 8 hours were washed with PBS followed by incubation for an additional 2 hours at 37°C in media containing 10 μg/ml BODIPY FL LDL. After washing with PBS, cells were fixed, and LDL binding/uptake was detected by fluorescence microscopy. ×375.
