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. 1998 Jul 7;95(14):8081–8086. doi: 10.1073/pnas.95.14.8081

Figure 1.

Figure 1

Reduction of NF-κB activity by IL-3 deprivation of Ba/F3 cells or inhibition of the TP kinase of Ba/F3.TP cells. (A) EMSA of nuclear extracts from IL-3-treated Ba/F3 cells or Ba/F3.TP cells. (B) EMSA of nuclear extracts prepared 18 h after IL-3-deprivation or CGP53716 treatment of Ba/F3 cells or Ba/F3.TP cells, respectively. (C) Cells transfected with pCMV-β-gal together with (Igκ3) conaluc or control conaluc vector were maintained in the presence or absence of IL-3 or 0.3 μM CGP53716. Luciferase activity was measured in cell extracts 4 h after transfection. Results, are the means ± SEM of three independent experiments and are expressed as % of the maximal activity.