Figure 2.

H5.110CMVhIFN-β inhibits the growth of established human colorectal cancer cell line tumors in an intrahepatic athymic nude mouse model for liver metastases and improves survival in an intrasplenic gross disease athymic nude mouse xenograft model of colorectal liver metastasis. (a) KM12L4 cells were directly injected in the livers of athymic nude mice. On day 14, when tumors were 3–5 mm³, animals were randomized to receive PBS, H5.010CMVβ-gal, or H5.110CMVhIFN-β via tail vein injection. On day 28 animals were sacrificed and sera and livers were harvested for tumor volume measurement. Each point represents a single animal. Mean tumor size is indicated by the large cross. Bars = SE. (P < 0.0001.) (b) KM12L4 cells were injected in the spleens of athymic nude mice followed by splenectomy. On day 21, mice were randomized to receive PBS, H5.010CMVβ-gal, or H5.110CMVhIFN-β via tail vein injection. Mice were sacrificed when they became moribund by preestablished criteria, and their survival curves were plotted.