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. 2001 Jul 1;108(1):143–151. doi: 10.1172/JCI11498

Figure 2.

Figure 2

TNF-induced activation of p42/p44 MAPK in human fibroblasts. Human fibroblasts transfected with empty vector (pcDNA3) or a vector encoding the truncated FAN (ΔFAN) were incubated for the indicated times in the presence or absence of human TNF (50 ng/ml). Expression of activated (dually phosphorylated; active MAPK) or total MAPK was visualized by Western blot (a). The data are representative of three independent experiments. (b) Quantification of p42/p44 MAPK activation by ImageQuant software (Molecular Dynamics, Sunnyvale, California, USA) (n = 3). The data for active MAPK were normalized to those for total MAPK.