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. 2001 Jul 1;108(1):143–151. doi: 10.1172/JCI11498

Figure 4.

Figure 4

TNF-induced cell death is impaired in fibroblasts from FAN-deficient mice. SV40-transformed fibroblasts from wild-type (FAN+/+) or FAN-knockout (FAN–/–) mice were incubated for 24 hours in the presence of the indicated concentrations of daunorubicin (DNR) (a), or for 72 hours under serum-free conditions with the indicated concentrations of human TNF and without cycloheximide (b). (c) Murine FAN-deficient fibroblasts were preincubated for 72 hours in the presence of an empty retroviral vector (FAN–/–) or a retroviral vector carrying the full-length FAN cDNA (FAN–/– corrected) and were then treated for 72 hours with 10 ng/ml of human TNF. Viability of FAN–/– and FAN–/– corrected cells was assessed by MTT assay (mean ± SE of three independent experiments) and expressed as percentage of the response observed in FAN+/+ cells (see b).