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. 2001 Jul 1;108(1):73–81. doi: 10.1172/JCI12466

Figure 6.

Figure 6

Metalloproteinase gene and AP-1 expression in JNK1 and JNK2 knockout (KO) FLS. Cultured FLS from JNK1 KO FLS lines, JNK2 KO FLS lines, and wild-type FLS lines were stimulated with medium or 2 ng/ml of IL-1 for 18 hours (Northern blot analysis) and 1 hour (EMSA) in the presence of medium or 20 μM SP600125. (a) MMP3 and MMP13 mRNA levels were determined by Northern blot analysis in two separate lines for each strain (line 1 and line 2). JNK1 KO and especially JNK2 KO FLS had lower MMP expression. Residual MMP expression was further suppressed by 20 μM SP600125 (see MMP3 experiment). The ethidium-stained gel shows equal loading of RNA in each lane. (b) AP-1 binding was shown for two JNK2 KO, one wild-type, and one JNK1 KO line. The far left part of the gel shows a positive control and cold competition of the positive control (cold oligo). JNK KO FLS lines have decreased basal and IL-1–stimulated AP-1 activation compared with wild-type cells.