Figure 2.
Effect of mepacrine on Ang II-induced AA release (A) and MAPK phosphorylation (B). (A) Serum-starved RTE cells were prelabeled with [3H]AA for 4 hr as described in Experimental Procedures, incubated with mepacrine (500 μM, 5 min), followed by stimulation with Ang II (1 μM, 5 min). The radioactivity in the extracellular medium was counted by liquid scintillation spectrometry. Data represent mean ± SEM from a single experiment performed in triplicate in parallel with measurement of MAPK phosphorylation. Two experiments had identical results. (B) RTE cells were preincubated with mepacrine as in A followed by stimulation with Ang II for 10 min. Cell lysates were analyzed by Western blotting with αPMAPK antibodies. Shown is a representation of three experiments with similar results.