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Effect of staurosporine on MAPK phosphorylation induced by PMA, AA, and Ang II. Serum-starved RTE cells were preincubated with different concentrations of staurosporine for 5 min, followed by stimulation with 1 μM PMA for 10 min (A), 15 μM AA for 5 min (B), or 1 μM Ang II for 10 min (C). Cell lysates were analyzed by Western blotting with αPMAPK antibodies. Shown is a representation of two independent experiments with similar results.
