Effect of AA, 5,6-EET, and 14,15-EET on MAPK activity. Serum-starved RTE cells were stimulated with 5,6-EET (10 μM) or 14,15-EET (10 μM) for 5 min, and MAPK activity was measured in MAPK immunoprecipitates as described in Experimental Procedures. Data represent the results of three experiments performed in duplicate. ∗, P < 0.05; ∗∗, P < 0.001.